全文获取类型
收费全文 | 36079篇 |
免费 | 4181篇 |
国内免费 | 1716篇 |
出版年
2023年 | 232篇 |
2022年 | 330篇 |
2021年 | 1103篇 |
2020年 | 831篇 |
2019年 | 997篇 |
2018年 | 1063篇 |
2017年 | 801篇 |
2016年 | 1157篇 |
2015年 | 1604篇 |
2014年 | 1929篇 |
2013年 | 2076篇 |
2012年 | 2423篇 |
2011年 | 2371篇 |
2010年 | 1410篇 |
2009年 | 1329篇 |
2008年 | 1545篇 |
2007年 | 1431篇 |
2006年 | 1333篇 |
2005年 | 1209篇 |
2004年 | 1016篇 |
2003年 | 962篇 |
2002年 | 822篇 |
2001年 | 2534篇 |
2000年 | 2347篇 |
1999年 | 1731篇 |
1998年 | 577篇 |
1997年 | 591篇 |
1996年 | 549篇 |
1995年 | 483篇 |
1994年 | 414篇 |
1993年 | 319篇 |
1992年 | 824篇 |
1991年 | 690篇 |
1990年 | 570篇 |
1989年 | 455篇 |
1988年 | 352篇 |
1987年 | 290篇 |
1986年 | 215篇 |
1985年 | 163篇 |
1984年 | 122篇 |
1983年 | 83篇 |
1982年 | 58篇 |
1981年 | 49篇 |
1980年 | 26篇 |
1979年 | 32篇 |
1978年 | 27篇 |
1976年 | 30篇 |
1974年 | 24篇 |
1973年 | 30篇 |
1970年 | 24篇 |
排序方式: 共有10000条查询结果,搜索用时 171 毫秒
991.
992.
993.
994.
Dormancy is a condition that delays or inhibits growth in seed, vegetative buds, and floral buds. In peach, seed germination occurs when seed accumulate sufficient stratification and growing degree hours to break dormancy and begin growing. Correlations have been reported between mean seed stratification requirements and mean bud chilling requirements among Prunus families, but an individual seed’s germination date and subsequent vegetative and floral bud break date are not correlated. Prior to this study, the genetic factors involved in regulating seed dormancy and their location on the peach genomic map were unknown. Segregating F2 seed were collected from a high?×?low chill F1 peach hybrid in 2005, 2006, and 2008. Germination date and growth habit was measured after the stratification requirement of the 2005 seed was fully met. The seed collected in 2006 and 2008 received varying amounts of stratification, which enabled data on stratification requirement, heat requirement, and growth habit to be collected. Genomic DNA was extracted from seedling leaf tissue and screened with SSR markers selected from the Prunus reference map at an average resolution of 20 cM. Seed dormancy quantitative trait loci (QTLs) were detected on G1, G4, G6/8, and G7. The QTLs detected on G6/8 and G7 were discovered in the same region as QTLs associated with floral bud chilling requirement and bloom time in peach. 相似文献
995.
996.
Fengjuan Jia Bingjiang Wu Hui Li Jinguang Huang Chengchao Zheng 《Molecular genetics and genomics : MGG》2013,288(11):559-577
F-box-containing proteins, as the key components of the protein degradation machinery, are widely distributed in higher plants and are considered as one of the largest known families of regulatory proteins. The F-box protein family plays a crucial role in plant growth and development and in response to biotic and abiotic stresses. However, systematic analysis of the F-box family in maize (Zea mays) has not been reported yet. In this paper, we identified and characterised the maize F-box genes in a genome-wide scale, including phylogenetic analysis, chromosome distribution, gene structure, promoter analysis and gene expression profiles. A total of 359 F-box genes were identified and divided into 15 subgroups by phylogenetic analysis. The F-box domain was relatively conserved, whereas additional motifs outside the F-box domain may indicate the functional diversification of maize F-box genes. These genes were unevenly distributed in ten maize chromosomes, suggesting that they expanded in the maize genome because of tandem and segmental duplication events. The expression profiles suggested that the maize F-box genes had temporal and spatial expression patterns. Putative cis-acting regulatory DNA elements involved in abiotic stresses were observed in maize F-box gene promoters. The gene expression profiles under abiotic stresses also suggested that some genes participated in stress responsive pathways. Furthermore, ten genes were chosen for quantitative real-time PCR analysis under drought stress and the results were consistent with the microarray data. This study has produced a comparative genomics analysis of the maize ZmFBX gene family that can be used in further studies to uncover their roles in maize growth and development. 相似文献
997.
998.
In eukaryotic cells short-lived proteins are degraded in a specific process by the ubiquitin-proteasome system (UPS), whereas long-lived proteins and damaged organelles are degraded by macroautophagy (hereafter referred to as autophagy). A growing body of evidence now suggests that autophagy is important for clearance of protein aggregates that form in cells as a consequence of ageing, oxidative stress, alterations that elevate the amounts of certain aggregation-prone proteins or expression of aggregating mutant variants of specific proteins. Autophagy is generally considered to be a non-specific, bulk degradation process. However, a recent study suggests that p62/SQSTM1 may link the recognition of polyubiquitinated protein aggregates to the autophagy machinery.1 This protein is able to polymerize via its N-terminal PB1 domain and to recognize polyubiquitin via its C-terminal UBA domain. It can also recruit the autophagosomal protein LC3 and co-localizes with many types of polyubiquitinated protein aggregates.1 Here we discuss possible implications of these findings and raise some questions for further investigation. 相似文献
999.
1000.
Xue Wang Fengting Xu Jiasen Liu Bingquan Gao Yanxin Liu Yujia Zhai Jun Ma Kai Zhang Timothy S. Baker Klaus Schulten Dong Zheng Hai Pang Fei Sun 《PLoS pathogens》2013,9(1)
Rabbit hemorrhagic disease, first described in China in 1984, causes hemorrhagic necrosis of the liver. Its etiological agent, rabbit hemorrhagic disease virus (RHDV), belongs to the Lagovirus genus in the family Caliciviridae. The detailed molecular structure of any lagovirus capsid has yet to be determined. Here, we report a cryo-electron microscopic (cryoEM) reconstruction of wild-type RHDV at 6.5 Å resolution and the crystal structures of the shell (S) and protruding (P) domains of its major capsid protein, VP60, each at 2.0 Å resolution. From these data we built a complete atomic model of the RHDV capsid. VP60 has a conserved S domain and a specific P2 sub-domain that differs from those found in other caliciviruses. As seen in the shell portion of the RHDV cryoEM map, which was resolved to ∼5.5 Å, the N-terminal arm domain of VP60 folds back onto its cognate S domain. Sequence alignments of VP60 from six groups of RHDV isolates revealed seven regions of high variation that could be mapped onto the surface of the P2 sub-domain and suggested three putative pockets might be responsible for binding to histo-blood group antigens. A flexible loop in one of these regions was shown to interact with rabbit tissue cells and contains an important epitope for anti-RHDV antibody production. Our study provides a reliable, pseudo-atomic model of a Lagovirus and suggests a new candidate for an efficient vaccine that can be used to protect rabbits from RHDV infection. 相似文献